*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Full length human TNF alpha recombinant protein
Affinity purified by Protein A.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
GTX637058 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX637058 WB Image
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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