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TNF alpha antibody [HL2363]

Recombinant antibody
Anti-TNF alpha antibody [HL2363] used in ELISA (ELISA). GTX638569
Anti-TNF alpha antibody [HL2363] used in ELISA (ELISA). GTX638569

Cat. No. GTX638569

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2363

Isotype

 IgG

Application

 ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX638569, Detection: GTX638567
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human TNF alpha recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F

Cellular Localization

Cell membrane

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TNF alpha antibody [HL2363] used in ELISA (ELISA). GTX638569

GTX638569 ELISA Image

Sandwich ELISA detection of recombinant 293T expressed, human Fc-tagged TNF alpha (54-233 a.a.) using antibodies as below.

Capture: TNF alpha antibody [HL2363] (GTX638569) (5 μg/mL)
Detection: HRP-conjugated TNF alpha antibody [HL2361] (GTX638567) (1 μg/mL)
Please notice that GTX638567 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638569. Please contact us for custom HRP-conjugated antibody.

Anti-TNF alpha antibody [HL2363] used in ELISA (ELISA). GTX638569

GTX638569 ELISA Image

Sandwich ELISA detection of recombinant 293T expressed, human Fc-tagged TNF alpha (54-233 a.a.) using antibodies as below.

Capture: TNF alpha antibody [HL2361] (GTX638567) (5 μg/mL)
Detection: HRP-conjugated TNF alpha antibody [HL2363] (GTX638569) (1 μg/mL)
Please notice that GTX638569 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638567. Please contact us for custom HRP-conjugated antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169