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TNF alpha antibody [MP6-XT22]

Anti-TNF alpha antibody [MP6-XT22] used in ELISA (ELISA). GTX75063

Cat. No. GTX75063

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MP6-XT22

Isotype

 IgG1

Application

 IHC-Fr, FACS, ELISA

Reactivity

 Mouse, Rat
Package
500 μg ($569)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
FACS 1/10-1/50
ELISA 1-10μg/ml

Note :

FACS
Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant murine TNF alpha expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_378345

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-a , TNF-alpha , TNFSF2 , TNFalpha , Tnfa , Tnfsf1a , Tnlg1f

Cellular Localization

Cell membrane; Single-pass type II membrane protein

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-TNF alpha antibody [MP6-XT22] used in ELISA (ELISA). GTX75063

GTX75063 ELISA Image

ELISA analysis of mouse TNF alpha recombinant protein using GTX75063 TNF alpha antibody [MP6-XT22].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 569