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TNF alpha antibody [MT11B10] (Biotin)

Anti-TNF alpha antibody [MT11B10] (Biotin) used in ELISA (ELISA). GTX03005-02

Cat. No. GTX03005-02

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MT11B10

Isotype

 IgG2a

Application

 ELISA, Sandwich ELISA

Reactivity

 Mouse
Package
250 μg ($399)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX03007, Detection: GTX03005-02.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Product Note

This antibody is able to detect native and recombinant mouse TNF-α.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse TNF-α

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane,Membrane,Secreted

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-TNF alpha antibody [MT11B10] (Biotin) used in ELISA (ELISA). GTX03005-02

GTX03005-02 ELISA Image

Sandwich ELISA analysis of mouse TNF-alpha protein using GTX03007 TNF alpha antibody [MT1C8 + 23C9] as coating antibody and GTX03005-02 TNF alpha antibody [MT11B10] (Biotin) as detecting antibody.
Substrate : pNPP

REFERENCE

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REVIEW

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Package List Price ($)
$ 399