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TNF alpha antibody [MT15B15] (Biotin)

Anti-TNF alpha antibody [MT15B15] (Biotin) used in ELISA (ELISA). GTX03006-02

Cat. No. GTX03006-02

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MT15B15

Isotype

 IgG1

Application

 WB, FACS, ELISA, Sandwich ELISA

Reactivity

 Human, Monkey
Package
250 μg ($399)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX03009, Detection: GTX03006-02.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Product Note

This antibody is able to detect native and recombinant human TNF-α.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human TNF-α

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DIF,TNF,TNFA,TNFSF2,TNFalpha,TNLG1F,tumor necrosis factor,TNF alpha

Cellular Localization

Cell membrane,Membrane,Secreted

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TNF alpha antibody [MT15B15] (Biotin) used in ELISA (ELISA). GTX03006-02

GTX03006-02 ELISA Image

Sandwich ELISA analysis of monkey TNF-alpha protein using GTX03009 TNF alpha antibody [MT21A8] as coating antibody and GTX03006-02 TNF alpha antibody [MT15B15] (Biotin) as detecting antibody.
Substrate : TMB

REFERENCE

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REVIEW

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Package List Price ($)
$ 399