APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Note :
Sandwich ELISA
Capture: GTX03010, Detection: GTX03008-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native and recombinant human TNF-α.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human TNF-α
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DIF,TNF,TNFA,TNFSF2,TNFalpha,TNLG1F,tumor necrosis factor,TNF alpha
Cellular Localization
Cell membrane,Membrane,Secreted
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX03010 ELISA Image
Sandwich ELISA analysis of human TNF-alpha protein using GTX03010 TNF alpha antibody [MT25C5] as coating antibody and GTX03008-02 TNF alpha antibody [MT20D9] (Biotin) as detecting antibody. Substrate : pNPP
|
REFERENCE
There are currently no references for TNF alpha antibody [MT25C5] (GTX03010). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TNF alpha antibody [MT25C5] (GTX03010). Be the first to share your experience with this product.