GeneTex
United States (US)

TNF alpha antibody [P/T2]

GTX35133_20170712_IHC-P_18121410_389.jpg

Cat No. GTX35133

Host Mouse
Clonality Monoclonal
Clone Name P/T2
Isotype IgM
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Zebrafish, Rabbit, Cat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 2-4ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF-a, conjugated to thyroglobulin

Purification

Ab purified from Bioreactor Concentrate

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Necrosis Factor,Dif,Tnf-Alpha,Tnfa,Tnfsf2,Tnlg1F,Tnf

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX35133_20170712_IHC-P_18121410_389.jpg

GTX35133 IHC-P Image

Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (P/T2).