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TNF alpha antibody [SPM543]

Anti-TNF alpha antibody [SPM543] used in IHC (Paraffin sections) (IHC-P). GTX35132

Cat. No. GTX35132

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM543

Isotype

 IgM

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit, Cat, Dog
Package
100 μg ($529)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A hexadecapeptide corresponding to aa115-130 of human TNF- , conjugated to thyroglobulin

Purification

Purified IgM

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DIF , TNF , TNFA , TNFSF2 , TNFalpha , TNLG1F , tumor necrosis factor , TNF alpha

Cellular Localization

Cell membrane,Membrane,Secreted

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TNF alpha antibody [SPM543] used in IHC (Paraffin sections) (IHC-P). GTX35132

GTX35132 IHC-P Image

IHC-P analysis of human histiocytoma tissue using GTX35132 TNF alpha antibody [SPM543].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529