GeneTex
United States (US)

TNF alpha antibody

Orthogonal Overexpression
GTX110520_41087_20151012_IHC-P_M_19011507_498.jpg
GTX110520_41087_20150917_WB_M_19011507_563.jpg
GTX110520_41087_20151012_IHC-P_R_19011507_287.jpg
GTX110520_41087_20150327_IHC_19011507_980.jpg
GTX110520_42536_20160630_WB_19011507_800.jpg
GTX110520_41087_20150917_WB_R_19011507_407.jpg
GTX110520_42536_20160817_IFA_19011507_271.jpg
GTX110520_42592_20170209_WB_M_LPS_19011507_121.jpg
GTX110520_41087_IHC_2_19011507_481.jpg
GTX110520_41087_IFA_19011507_262.jpg
GTX110520_41087_WB_2_19011507_341.jpg
GTX110520_42592_20181207_WB_B_19011519_122.jpg

Cat No. GTX110520

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Raji , NCI-H929 , C8D30 , PC-12 , Rat-2 , Raw264.7 (50 ng/ml LPS treatment for 4 hr) , TNF-transfected 293T

Predict Reactivity

Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human TNF alpha Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, TNF antibody, TNF, monocyte-derived antibody, TNF, macrophage-derived antibody, APC1 protein antibody, cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor-alpha antibody, tumor necrosis factor alpha antibody, TNF-a antibody, tumor necrosis factor ligand superfamily member 2 antibody, TNF superfamily, member 2 antibody

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Tumor necrosis factor , soluble form: Secreted

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX110520_41087_20151012_IHC-P_M_19011507_498.jpg

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110520_41087_20150917_WB_M_19011507_563.jpg

GTX110520 WB Image

TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110520_41087_20151012_IHC-P_R_19011507_287.jpg

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110520_41087_20150327_IHC_19011507_980.jpg

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma .
TNF alpha antibody (GTX110520) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110520_42536_20160630_WB_19011507_800.jpg

GTX110520 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110520_41087_20150917_WB_R_19011507_407.jpg

GTX110520 WB Image

TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110520_42536_20160817_IFA_19011507_271.jpg

GTX110520 ICC/IF Image

TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.
Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX110520_42592_20170209_WB_M_LPS_19011507_121.jpg

GTX110520 WB Image

Orthogonal

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000.

GTX110520_41087_IHC_2_19011507_481.jpg

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
TNF alpha antibody (GTX110520) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX110520_41087_IFA_19011507_262.jpg

GTX110520 ICC/IF Image

TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.
Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX110520_41087_WB_2_19011507_341.jpg

GTX110520 WB Image

TNF alpha antibody detects TNF alpha protein by western blot analysis.
A. 30 µg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
TNF alpha antibody (GTX110520) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110520_42592_20181207_WB_B_19011519_122.jpg

GTX110520 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf