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TNF alpha antibody

Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520
Anti-TNF alpha antibody used in Western Blot (WB). GTX110520
Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520
Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520
Anti-TNF alpha antibody used in Western Blot (WB). GTX110520
Anti-TNF alpha antibody used in Western Blot (WB). GTX110520
Anti-TNF alpha antibody used in Western Blot (WB). GTX110520
Anti-TNF alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110520
Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520
Anti-TNF alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110520
Anti-TNF alpha antibody used in Western Blot (WB). GTX110520
Anti-TNF alpha antibody used in Western Blot (WB). GTX110520

Cat No. GTX110520

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat, Bovine
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

TNF alpha antibody recognizes tumor necrosis factor alpha protein, a cytokine with a predicted molecular weight of 26 kDa. TNF alpha is mainly secreted by macrophages, but is also produced by various immune cells such as neutrophils, T helper cells, and NK cells. Dysregulation of TNF alpha expression is associated with various diseases including cancers and autoimmune disorders.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

Raji , NCI-H929 , C8D30 , PC-12 , Rat-2 , Raw264.7 (50 ng/ml LPS treatment for 4 hr) , TNF-transfected 293T

Predict Reactivity

Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human TNF alpha Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885286

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Tumor necrosis factor , soluble form: Secreted

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TNF alpha antibody used in Western Blot (WB). GTX110520

GTX110520 WB Image

TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma .
TNF alpha antibody (GTX110520) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TNF alpha antibody used in Western Blot (WB). GTX110520

GTX110520 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TNF alpha antibody used in Western Blot (WB). GTX110520

GTX110520 WB Image

TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TNF alpha antibody used in Western Blot (WB). GTX110520

GTX110520 WB Image

TNF alpha antibody detects TNF alpha protein by western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
TNF alpha antibody (GTX110520) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TNF alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110520

GTX110520 ICC/IF Image

TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.
Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-TNF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX110520

GTX110520 IHC-P Image

TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
TNF alpha antibody (GTX110520) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TNF alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110520

GTX110520 ICC/IF Image

TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.
Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-TNF alpha antibody used in Western Blot (WB). GTX110520

GTX110520 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TNF alpha antibody used in Western Blot (WB). GTX110520

GTX110520 WB Image

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

TNF alpha antibody Cat No. GTX110520

Rating ( Average 4.9 based on 7 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Neurol in 2017.PMID: 28713327
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2017.PMID: 29184499
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 31757960
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 31757960
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Bov
Figure Note : The data was peer reviewed and published in the journal Cardiovasc Diabetol in 2014.PMID: 24666525
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 30987270
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colonic mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf