PRODUCT
Summary
TNF alpha antibody recognizes tumor necrosis factor alpha protein, a cytokine with a predicted molecular weight of 26 kDa. TNF alpha is mainly secreted by macrophages, but is also produced by various immune cells such as neutrophils, T helper cells, and NK cells. Dysregulation of TNF alpha expression is associated with various diseases including cancers and autoimmune disorders.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
NCI-H929 , C8D30 , PC-12 , Rat-2 , Raji , TNF-transfected 293T
Predict Reactivity
Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.28 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human TNF alpha Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885286
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
Cellular Localization
Cell membrane; Single-pass type II membrane protein , Tumor necrosis factor , soluble form: Secreted
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110520 WB Image
TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 WB Image
TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 WB Image
Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 WB Image
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110520 ICC/IF Image
TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis. Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX110520 ICC/IF Image
TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis. Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500. Blue: Hoechst 33342 staining.
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REFERENCE
REVIEW
TNF alpha antibody
Cat. No. GTX110520
Rating
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( Average 4.9 based on 11 users reviews)
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Application
Western Blot(WB)
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( Average 4.9 based on 8 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 03-Oct-2022
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the 2022 in Int J Mol Sci.
PMID: 35628464
Date :
Anonymous submitted on 03-Oct-2022
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the 2022 in Int J Mol Sci.
PMID: 35628464
Date :
Anonymous submitted on 03-Oct-2022
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the 2022 in Int J Mol Sci.
PMID: 35628464
Date :
Anonymous submitted on 03-Oct-2022
Application Tested :
IHC-P
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the 2022 in PLoS One.
PMID: 35303036
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Neurol in 2017.
PMID: 28713327
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC-P
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Front Pharmacol in 2017.
PMID: 29184499
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2019.
PMID: 31757960
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Nat Commun in 2019.
PMID: 31757960
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Bov
Figure Note :
The data was peer reviewed and published in the journal Cardiovasc Diabetol in 2014.
PMID: 24666525
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Int J Mol Sci in 2019.
PMID: 30987270
Date :
Anonymous submitted on 22-Feb-2021
Application Tested :
WB
Sample Species :
Ms
Sample :
colonic mucosa
Amount used :
25 μg
Blocking :
5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr