APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| Assay dependent |
| 1:100-1:500 |
| 1:1000-1:5000 |
Not tested in other applications.
Calculated MW
Product Note
The antibody does not recognize human TNFβ (lymphotoxin).
PROPERTIES
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human TNF alpha
Purification
IgG fraction
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
Cellular Localization
Cell membrane
Background
Tumor necrosis factor-alpha (TNF-a), also known as cachectin, is an inflammatory. TNF-a is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, Astrocytes, endothelial cells, smooth muscle cells and some transformed cells. TNF-a appears to be a major mediator of inflammatory responses and overproduction results in the extensive tissue damage associated with endotoxemia and cachexia. In addition, high serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections and various neoplastic diseases. TNF-a influences the growth and function of both normal and neoplastic cells. On normal cells, TNF-a exhibits growth enhancing activities for fibroblasts, T cells and B cells; differentiation of myeloid cells. On neoplastic cells, TNF-a exerts growth inhibitory activities that can be either cytostatic or cytotoxic.
Database
Research Area
DATA IMAGES
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GTX26671 WB Image
GTX26671 was used at a 1:1000 dilution incubated o/n 4ºC to detect recombinant human TNF-Alpha protein by Western blot.
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GTX26671 IHC-P Image
Fluorescent IHC-P staining of human colon using anti-TNF alpha antibody (GTX26671) at a primary antibody dilution of 1:100.
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GTX26671 IHC-P Image
Immunohistochemistry using GTX26671 showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections.
Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4ºC. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
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GTX26671 IHC-P Image
IHC-P analysis of human artery tissue using GTX26671 TNF alpha antibody.
Antigen retrieval : Heat mediated citrate buffer (pH 6.0)
Dilution : 1:100
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GTX26671 IHC-P Image
IHC-P analysis of human colon tissue using GTX26671 TNF alpha antibody.
Dilution : 1:100
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GTX26671 WB Image
WB analysis of various samples using GTX26671 TNF alpha antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : LNCap whole cell lysate
Lane 8 : Molt-4 whole cell lysate
Lane 9 : Ramos whole cell lysate
Lane 10 : Raji whole cell lysate
Lane 11 : A-172 whole cell lysate
Lane 12 : NIH-3T3 whole cell lysate
Loading : 35 μg
Dilution : 1 μg/mL
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REFERENCE
REVIEW
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