For FACS, IA, and WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
The antibody can be used for neutralization of human and monkey TNF-alpha in bio-assays.
Phosphate Buffered Saline containing 0.1% BSA and 0.02% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Tumor Necrosis Factor,Dif,Tnf-Alpha,Tnfa,Tnfsf2,Tnlg1F,Tnf
Type 2 membrane protein or soluble form derived from the membrane by proteolytic cleavage.
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.