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United States (US)

TNF alpha antibody

Cat. No. GTX74120

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Functional Assay

Reactivity

 Rat
Package
100 μg ($619)
See all TNF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.2μg/ml
IHC-P Assay dependent
ELISA 0.5-2μg/ml
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant rat TNF alpha

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_379059

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor necrosis factor , RATTNF , TNF-alpha , Tnfa

Cellular Localization

Cell membrane

Background

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

Database

Research Area

REFERENCE

Application Reference

Sarfraz Ahmed et al. J Oncol 2022; 2022 : 6457266 Evaluation of Effect of Honey Sugars Analogue Therapy against Breast Cancer Induced by 1-Methyl-1-nitrosourea in In Vivo Breast Cancer Model.
Application : IHC-P
Reactivity : Rat
Ahmed S et al. Evid Based Complement Alternat Med 2017; 2017 : 5904361 Oral Administration of Tualang and Manuka Honeys Modulates Breast Cancer Progression in Sprague-Dawley Rats Model.
Zhao J et al. Sci Rep 2016; 6 : 19670 the c-jun n-terminal kinase (jnk) pathway is activated in human interstitial cystitis (ic) and rat * sulfate induced cystitis.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Package List Price ($)
$ 619