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TNF beta antibody

Anti-TNF beta antibody used in Western Blot (WB). GTX130100
Anti-TNF beta antibody used in Western Blot (WB). GTX130100
Anti-TNF beta antibody used in Western Blot (WB). GTX130100

Cat No. GTX130100

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

human spleen , rat spleen

Predict Reactivity

Dog, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TNF beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lymphotoxin alpha , LT , TNFB , TNFSF1 , TNLG1E

Cellular Localization

Secreted , Membrane

Background

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TNF beta antibody used in Western Blot (WB). GTX130100

GTX130100 WB Image

TNF beta antibody detects TNF beta protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF beta antibody (GTX130100) diluted at 1:500.

Anti-TNF beta antibody used in Western Blot (WB). GTX130100

GTX130100 WB Image

TNF beta antibody detects TNF beta protein by western blot analysis. Rat tissue extracts (30 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with TNF beta antibody (GTX130100) at a dilution of 1:500.

Anti-TNF beta antibody used in Western Blot (WB). GTX130100

GTX130100 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF beta antibody (GTX130100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169