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TNFAIP2 antibody [C1C3]

Anti-TNFAIP2 antibody [C1C3] used in Western Blot (WB). GTX105256
Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256
Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256
Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256
Anti-TNFAIP2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105256
Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256

Cat. No. GTX105256

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TNFAIP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TNFAIP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038142

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF alpha induced protein 2 , B94 , EXOC3L3

Background

This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. The expression of this gene was shown to be induced by retinoic acid in a cell line expressing a oncogenic version of the retinoic acid receptor alpha fusion protein, which suggested that this gene may be a retinoic acid target gene in acute promyelocytic leukemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TNFAIP2 antibody [C1C3] used in Western Blot (WB). GTX105256

GTX105256 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TNFAIP2 antibody [C1C3] (GTX105256) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256

GTX105256 IHC-P Image

TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
TNFalpha-IP 2 antibody [C1C3] (GTX105256) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256

GTX105256 IHC-P Image

TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
TNFalpha-IP 2 antibody [C1C3] (GTX105256) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256

GTX105256 IHC-P Image

TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
TNFalpha-IP 2 antibody [C1C3] (GTX105256) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TNFAIP2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105256

GTX105256 ICC/IF Image

TNFAIP2 antibody [C1C3] detects TNFAIP2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNFAIP2 stained by TNFAIP2 antibody [C1C3] (GTX105256) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TNFAIP2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105256

GTX105256 IHC-P Image

TNFalpha-IP 2 antibody [C1C3] detects TNFalpha-IP 2 protein at cytosol on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
TNFalpha-IP 2 antibody [C1C3] (GTX105256) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169