APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
TNFRSF14-transfected 293T , RD , Molt-4 , mouse spleen
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.78 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TNFRSF14. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11168267
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TNF receptor superfamily member 14 , ATAR , CD270 , HVEA , HVEM , LIGHTR , TR2
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/templates/default/js/lightbox/images/loading.gif "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
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GTX113589 WB Image
Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TNFRSF14 antibody [N2C3] (GTX113589) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113589 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TNFRSF14 antibody [N2C3] (GTX113589) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113589 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TNFRSF14 antibody [N2C3] (GTX113589) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113589 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TNFRSF14 antibody [N2C3] (GTX113589) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for TNFRSF14 antibody [N2C3] (GTX113589). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TNFRSF14 antibody [N2C3] (GTX113589). Be the first to share your experience with this product.
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/small/GTX113589/GTX113589_42851_20171103_WB_M_spleen_w_23060501_184.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/small/GTX113589/GTX113589_42851_20170922_WB_B_w_23060501_850.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/small/GTX113589/GTX113589_42851_20171103_WB_w_23060501_767.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/small/GTX113589/GTX113589_42851_20171103_WB_2_w_23060501_193.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/normal/GTX113589/GTX113589_42851_20171103_WB_M_spleen_w_23060501_184.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/normal/GTX113589/GTX113589_42851_20170922_WB_B_w_23060501_850.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/normal/GTX113589/GTX113589_42851_20171103_WB_w_23060501_767.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")
![Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589](/upload/website/prouct_img/normal/GTX113589/GTX113589_42851_20171103_WB_2_w_23060501_193.webp "Anti-TNFRSF14 antibody [N2C3] used in Western Blot (WB). GTX113589")