TNIK antibody

Cat. No. GTX13141

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	25 μl ($399)

See all TNIK products

View product citations for antibody GTX13141 on CiteAb

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

155 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_383061

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TRAF2 and NCK interacting kinase , MRT54

Background

Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM]

Database

Gene ID: 23043 TNIK
UniProt: Q9UKE5 TNIK

Research Area

DATA IMAGES

GTX13141 IHC-P Image

TNIK antibody detects TNIK protein at cytosol on Mouse small intestine by immunohistochemical analysis.
Sample: Paraffin-embedded Mouse small intestine
TNIK antibody (GTX13141) dilution: 1:100
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX13141 ICC/IF Image

TNIK antibody detects TNIK protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNIK protein stained by TNIK antibody (GTX13141) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX13141 WB Image

TNIK antibody validation by shRNA knock-down
Upper panel: TNIK antibody (GTX13141)
Lower panel: p84 antibody(GTX70220)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TNIK shRNA-transfected 293T cells
5% SDS-PAGE
TNIK antibody (GTX13141) dilution: 1:5000
p84 antibody(GTX70220) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX13141 IHC-P Image

TNIK antibody detects TNIK protein at cytosol on Mouse Kidney by immunohistochemical analysis.
Sample: Paraffin-embedded Mouse Kidney
TNIK antibody (GTX13141) dilution: 1:100
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX13141 IHC-P Image

TNIK antibody detects TNIK protein at cytosol on mouse Colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse Colon
TNIK antibody (GTX13141) dilution: 1:100
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min