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TNR antibody [C1C2], Internal

Anti-TNR antibody [C1C2], Internal used in Western Blot (WB). GTX118246
Anti-TNR antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118246

Cat. No. GTX118246

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TNR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

150 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TNR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618054

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tenascin R , TN-R

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Tenascin-R (TNR) is an extracellular matix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion; MIM 187380), TNX (TNXB; MIM 600985), and TNR (Erickson, 1993 [PubMed 7694605]). The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TNR antibody [C1C2], Internal used in Western Blot (WB). GTX118246

GTX118246 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TNR antibody [C1C2], Internal (GTX118246) diluted at 1:500.

Anti-TNR antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118246

GTX118246 ICC/IF Image

TNR antibody [C1C2], Internal detects TNR protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNR stained by TNR antibody [C1C2], Internal (GTX118246) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169