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TOE1 antibody

Anti-TOE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117545
Anti-TOE1 antibody used in Western Blot (WB). GTX117545
Anti-TOE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117545

Cat. No. GTX117545

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HCT116

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TOE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162986

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

target of EGR1, exonuclease , PCH7 , hCaf1z

Cellular Localization

Nucleus , nucleolus , Nucleus speckle

Background

Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1.

Database

Research Area

DATA IMAGES

Anti-TOE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117545

GTX117545 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TOE1(GTX117545) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TOE1 antibody used in Western Blot (WB). GTX117545

GTX117545 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX117545 diluted at 1:1000

Anti-TOE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117545

GTX117545 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TOE1(GTX117545) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169