Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TOE1(GTX117545) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30 ug of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX117545 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TOE1(GTX117545) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min