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TOM1L1 antibody [3F12]

Orthogonal Overexpression
Anti-TOM1L1 antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632246
Anti-TOM1L1 antibody [3F12] used in Western Blot (WB). GTX632246
Anti-TOM1L1 antibody [3F12] used in Western Blot (WB). GTX632246

Cat. No. GTX632246

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F12

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TOM1L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

human TOM1L1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human TOM1L1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2888299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

target of myb1 like 1 membrane trafficking protein , SRCASM

Cellular Localization

Golgi apparatus , Golgi stack , Endosome membrane

Background

Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promotes FYN activation, possibly by disrupting intramolecular SH3-dependent interactions.

Database

Research Area

DATA IMAGES

Anti-TOM1L1 antibody [3F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632246

GTX632246 ICC/IF Image

Orthogonal

ICC/IF analysis of EGF stimulated A431 cells using GTX632246 TOM1L1 antibody [3F12].
Red : Primary antibody
Green : Polyclonal TOM1L1 antibody
Yellow : Merged color of red and green

Anti-TOM1L1 antibody [3F12] used in Western Blot (WB). GTX632246

GTX632246 WB Image

Overexpression

WB analysis of HeLa lysate transfected with TOM1L1 using GTX632246 TOM1L1 antibody [3F12].
T : Transfected HeLa with HA-TOM1L1 fL
L : HeLa lysate

Anti-TOM1L1 antibody [3F12] used in Western Blot (WB). GTX632246

GTX632246 WB Image

Overexpression

TOM1L1 antibody detects TOM1L1 protein by western blot analysis. Non-transfected (-) and TOM1L1-transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOM1L1 antibody (GTX632246) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations