GeneTex
United States (US)

TOM1L1 antibody [C3], C-term

Overexpression
GTX106377_WB_19011506_306.jpg
GTX106377_39694_20180608_WB_B_19011506_860.jpg
GTX106377_39694_IFA_19011506_819.jpg

Cat No. GTX106377

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Molt-4 , Raji , TOM1L1-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TOM1L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

OK/KNS-CL.3 antibody, SRCASM antibody, TOM1L1 antibody, target of myb 1-like 1 antibody, target of myb1-like 1 antibody, TOM1-like protein 1 antibody, src-activating and signaling molecule protein antibody, target of Myb-like protein 1 antibody, Src activating and signaling molecule antibody, target of myb1 (chicken)-like 1 antibody

Cellular Localization

Golgi apparatus , Golgi stack , Endosome membrane

Background

Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promotes FYN activation, possibly by disrupting intramolecular SH3-dependent interactions.

Database

DATA IMAGES
GTX106377_WB_19011506_306.jpg

GTX106377 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX106377 diluted at 1:3000

GTX106377_39694_20180608_WB_B_19011506_860.jpg

GTX106377 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOM1L1 antibody [C3], C-term (GTX106377) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106377_39694_IFA_19011506_819.jpg

GTX106377 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using TOM1L1(GTX106377) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169