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TOM1L1 antibody [C3], C-term

Overexpression
Anti-TOM1L1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106377
Anti-TOM1L1 antibody [C3], C-term used in Western Blot (WB). GTX106377
Anti-TOM1L1 antibody [C3], C-term used in Western Blot (WB). GTX106377

Cat. No. GTX106377

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TOM1L1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Raji , TOM1L1-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TOM1L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241401

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

target of myb1 like 1 membrane trafficking protein , SRCASM

Cellular Localization

Golgi apparatus , Golgi stack , Endosome membrane

Background

Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promotes FYN activation, possibly by disrupting intramolecular SH3-dependent interactions.

Database

Research Area

DATA IMAGES

Anti-TOM1L1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106377

GTX106377 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using TOM1L1(GTX106377) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-TOM1L1 antibody [C3], C-term used in Western Blot (WB). GTX106377

GTX106377 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOM1L1 antibody [C3], C-term (GTX106377) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOM1L1 antibody [C3], C-term used in Western Blot (WB). GTX106377

GTX106377 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX106377 diluted at 1:3000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169