GeneTex
United States (US)

TOM1L1 antibody [N2C2], Internal

GTX115333_40688_WB_18121410_943.jpg
GTX115333_40688_IHC_18121410_215.jpg
GTX115333_40688_20180608_WB_B_18121410_494.jpg

Cat No. GTX115333

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A431 , HeLa , TOM1L1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TOM1L1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Target Of Myb1 Like 1 Membrane Trafficking Protein,Srcasm,Tom1L1

Cellular Localization

Golgi apparatus , Golgi stack , Endosome membrane

Background

Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promotes FYN activation, possibly by disrupting intramolecular SH3-dependent interactions.

Database

DATA IMAGES
GTX115333_40688_WB_18121410_943.jpg

GTX115333 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
10% SDS PAGE
GTX115333 diluted at 1:1000

GTX115333_40688_IHC_18121410_215.jpg

GTX115333 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using TOM1L1(GTX115333) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115333_40688_20180608_WB_B_18121410_494.jpg

GTX115333 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOM1L1 antibody [N2C2], Internal (GTX115333) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169