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TOM1L2 antibody, C-term

Anti-TOM1L2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89650
Anti-TOM1L2 antibody, C-term used in Western Blot (WB). GTX89650

Cat. No. GTX89650

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all TOM1L2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-1μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Skeletal Muscle shows variable staining from fibre to fibre.
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89650-PEP).

Product Note

This antibody is expected to recognise both reported isoforms.

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CRKKPERSEDALFAL, from the C Terminus of the protein sequence according to NP_001028723.1; NP_001076437.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

target of myb1 like 2 membrane trafficking protein

Background

This gene belongs to a small gene family whose members have an N-terminal VHS domain followed by a GAT domain; domains which typically participate in vesicular trafficking. The canonical protein encoded by this gene also has a C-terminal clathrin binding motif. This protein has been shown to interact with Tollip, clathrin and ubiquitin and is thought to play a role in endosomal sorting. This gene resides in the 3.7 Mb deletion of chromosome region 17p11.2 that is associated with Smith-Magenis syndrome. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Apr 2017]

Database

Research Area

DATA IMAGES

Anti-TOM1L2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89650

GTX89650 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX89650 TOM1L2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-TOM1L2 antibody, C-term used in Western Blot (WB). GTX89650

GTX89650 WB Image

WB analysis of A431 lysate using GTX89650 TOM1L2 antibody, C-term.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399