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TOMM20 antibody

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

Cat No. GTX133756

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length Human TOMM20 recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

translocase of outer mitochondrial membrane 20 , MAS20 , MOM19 , TOM20

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein

Background

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

Database

Research Area

DATA IMAGES

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (GTX133756) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (GTX133756) diluted at 1:1000 and competitor's antibody (sc-11415) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169