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TOMM20 antibody

Comparison Orthogonal
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756

Cat. No. GTX133756

Reference 3
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TOMM20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse kidney , rat thymus

Predict Reactivity

Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length Human TOMM20 recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887094

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

translocase of outer mitochondrial membrane 20 , MAS20 , MOM19 , TOM20

Cellular Localization

Mitochondrion outer membrane; Single-pass membrane protein

Background

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

Database

Research Area

DATA IMAGES

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (GTX133756) diluted at 1:1000 and competitor's antibody (Competitor A) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:615.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with TOMM20 antibody (GTX133756) diluted at 1:1000 and competitor's antibody (sc-11415) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756

GTX133756 ICC/IF Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-TOMM20 antibody used in Western Blot (WB). GTX133756

GTX133756 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody (GTX133756) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX133756

GTX133756 IHC-P Image

TOMM20 antibody detects TOMM20 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756

GTX133756 ICC/IF Image

TOMM20 antibody detects TOMM20 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TOMM20 stained by TOMM20 antibody (GTX133756) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Chen KC et al. J Clin Med 2021; 11 (1) Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics.
Application : WB
Reactivity : Rat
Bosc C et al. Nat Commun 2020; 11 (1) : 4056 Autophagy regulates fatty acid availability for oxidative phosphorylation through mitochondria-endoplasmic reticulum contact sites.
Application : ICC/IF
Reactivity : Human
Keller G et al. Neurobiol Dis 2018; 124 : 57-66 Mitochondrial dysfunction in preclinical genetic prion disease: A target for preventive treatment?
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

TOMM20 antibody Cat. No. GTX133756

Rating
( Average 4.8 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Date : Anonymous submitted on 12-Nov-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse Brain tissue
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:500, 25°C, 16Hr
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133756
Date : Anonymous submitted on 03-May-2023
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HCT116 cells
Blocking : PBS, 2% BSA, 2% FBS, 0.1% Tween20, 0.02% NaN3, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100
Primary Antibody : 1:200, 25°C, 1Hr
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Date : Anonymous submitted on 14-Oct-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U937 cells
Amount used : 80μg
Blocking : PVDF Blocking reagent, RT, 1Hr
Primary Antibody : 1:3000, 4°C, > 12Hr
Anti-TOMM20 antibody used in Western Blot (WB). GTX133756
Date : Anonymous submitted on 12-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Synapotosome isolated from mouse brain tissue
Amount used : 10-20 μg
Blocking : 3% milk powder in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Figure Note : Numbers indicated separate mice; L: left and R: right hippocampus and prefrontal cortex (PFC).
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169