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TOMM20 antibody

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32928
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32928
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX32928
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX32928
Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX32928
Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32928
Anti-TOMM20 antibody used in Immunoprecipitation (IP). GTX32928
Anti-TOMM20 antibody used in Western Blot (WB). GTX32928

Cat. No. GTX32928

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TOMM20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOMM20 (NP_055580.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2734125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

translocase of outer mitochondrial membrane 20 , MAS20 , MOM19 , TOM20

Cellular Localization

Mitochondrion outer membrane

Database

Research Area

DATA IMAGES

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32928

GTX32928 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32928 TOMM20 antibody.
Blue : DAPI
Dilution : 1:100

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32928

GTX32928 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32928 TOMM20 antibody.
Blue : DAPI
Dilution : 1:100

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX32928

GTX32928 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX32928 TOMM20 antibody.
Dilution : 1:100

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX32928

GTX32928 IHC-P Image

IHC-P analysis of human colon tissue using GTX32928 TOMM20 antibody.
Dilution : 1:100

Anti-TOMM20 antibody used in IHC (Paraffin sections) (IHC-P). GTX32928

GTX32928 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX32928 TOMM20 antibody.
Dilution : 1:100

Anti-TOMM20 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32928

GTX32928 ICC/IF Image

ICC/IF analysis of L929 cells using GTX32928 TOMM20 antibody.
Blue : DAPI
Dilution : 1:100

Anti-TOMM20 antibody used in Immunoprecipitation (IP). GTX32928

GTX32928 IP Image

IP analysis of HeLa cell lysate using GTX32928 TOMM20 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-TOMM20 antibody used in Western Blot (WB). GTX32928

GTX32928 WB Image

WB analysis of various sample lysates using GTX32928 TOMM20 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Hutchison V et al. J Cell Biol 2024; 223 (1) Inducible tricolor reporter mouse for parallel imaging of lysosomes, mitochondria, and microtubules.
Brown MR et al. J Clin Invest 2021; 131 (17) Electrogenic sodium bicarbonate cotransporter NBCe1 regulates pancreatic β cell function in type 2 diabetes.
Application : IHC-P
Reactivity : Mouse
Choi BW et al. Nucl Med Mol Imaging 2019; 53 (6) : 396-405 Reverse Warburg Effect-Related Mitochondrial Activity and 18F-FDG Uptake in Invasive Ductal Carcinoma.
Galvan DL et al. J Clin Invest 2019; 130 Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399