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TOMM22 antibody

Anti-TOMM22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134799
Anti-TOMM22 antibody used in Western Blot (WB). GTX134799

Cat. No. GTX134799

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TOMM22 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TOMM22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887347

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

translocase of outer mitochondrial membrane 22 , 1C9-2 , MST065 , MSTP065 , TOM22

Cellular Localization

Mitochondrion outer membrane

Background

The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TOMM22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134799

GTX134799 ICC/IF Image

TOMM22 antibody detects TOMM22 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TOMM22 stained by TOMM22 antibody (GTX134799) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-TOMM22 antibody used in Western Blot (WB). GTX134799

GTX134799 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TOMM22 antibody (GTX134799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169