APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.34 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TOMM40. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887107
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
translocase of outer mitochondrial membrane 40 , C19orf1 , D19S1177E , PER-EC1 , PEREC1 , TOM40
Cellular Localization
Mitochondrion outer membrane; Multi-pass membrane protein
Background
TOMM40 is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for protein import into mitochondria (Humphries et al., 2005 [PubMed 15644312]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX133780 WB Image
HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with TOMM40 antibody (GTX133780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133780 IHC-P Image
TOMM40 antibody detects TOMM40 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.TOMM40 stained by TOMM40 antibody (GTX133780) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133780 IHC-P Image
TOMM40 antibody detects TOMM40 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.TOMM40 stained by TOMM40 antibody (GTX133780) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133780 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TOMM40 antibody (GTX133780) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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