TOP1MT antibody [TOP1MT/488]

Cat. No. GTX35136

Host	Mouse
Clonality	Monoclonal
Clone Name	TOP1MT/488
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

See all TOP1MT products

Datasheet
Document

View product citations for antibody GTX35136 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human TOP1MT protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TOP1MT , topoisomerase (DNA) I, mitochondrial , DNA topoisomerase I mitochondrial

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Gene ID: 116447 TOP1MT
UniProt: Q969P6 TOP1MT

Research Area

DATA IMAGES

GTX35136 WB Image

WB analysis of Jurkat cell lysate using GTX35136 TOP1MT antibody [TOP1MT/488].

GTX35136 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX35136 TOP1MT antibody [TOP1MT/488].

GTX35136 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX35136 TOP1MT antibody [TOP1MT/488].

GTX35136 FACS Image

FACS analysis of PFA-fixed MCF-7 cells using GTX35136 TOP1MT antibody [TOP1MT/488].
Blue : Primary antibody
Red : Isotype control

GTX35136 IHC-P Image

IHC-P analysis of human gallbladder tissue using GTX35136 TOP1MT antibody [TOP1MT/488].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty

TOP1MT antibody [TOP1MT/488]

Cat. No. GTX35136

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX35136 WB Image

GTX35136 IHC-P Image

GTX35136 IHC-P Image

GTX35136 FACS Image

GTX35136 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.