GeneTex
United States (US)

TOP1MT antibody [TOP1MT/488]

Anti-TOP1MT antibody [TOP1MT/488] used in IHC (Paraffin sections) (IHC-P). GTX35136
Anti-TOP1MT antibody [TOP1MT/488] used in IHC (Paraffin sections) (IHC-P). GTX35136
Anti-TOP1MT antibody [TOP1MT/488] used in IHC (Paraffin sections) (IHC-P). GTX35136

Cat No. GTX35136

Host Mouse
Clonality Monoclonal
Clone Name TOP1MT/488
Isotype IgG2b
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Specificity/Sensitivity

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human TOP1MT protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Topoisomerase I Mitochondrial,Top1Mt

Background

This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES
Anti-TOP1MT antibody [TOP1MT/488] used in IHC (Paraffin sections) (IHC-P). GTX35136

GTX35136 IHC-P Image

Formalin-fixed, paraffin-embedded human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).

Anti-TOP1MT antibody [TOP1MT/488] used in IHC (Paraffin sections) (IHC-P). GTX35136

GTX35136 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).

Anti-TOP1MT antibody [TOP1MT/488] used in IHC (Paraffin sections) (IHC-P). GTX35136

GTX35136 IHC-P Image

Formalin-fixed, paraffin-embedded human Pancreas stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).

Package List Price ($)
$ 329