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TOPBP1 antibody

Anti-TOPBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16438
Anti-TOPBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX16438
Anti-TOPBP1 antibody used in Western Blot (WB). GTX16438

Cat. No. GTX16438

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all TOPBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

171 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA topoisomerase II binding protein 1 , Dpb11 , TOP2BP1

Cellular Localization

Nucleus

Background

This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TOPBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16438

GTX16438 ICC/IF Image

ICC/IF analysis of A549 cells using GTX16438 TOPBP1 antibody.
Blue : DAPI

Anti-TOPBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX16438

GTX16438 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX16438 TOPBP1 antibody.
Dilution : 1:100

Anti-TOPBP1 antibody used in Western Blot (WB). GTX16438

GTX16438 WB Image

WB analysis of 293T cell lysate using GTX16438 TOPBP1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399