GeneTex
United States (US)

TOX3 antibody [TOX3/1123]

Anti-TOX3 antibody [TOX3/1123] used in IHC (Paraffin sections) (IHC-P). GTX35139
Anti-TOX3 antibody [TOX3/1123] used in IHC (Paraffin sections) (IHC-P). GTX35139
Anti-TOX3 antibody [TOX3/1123] used in IHC (Paraffin sections) (IHC-P). GTX35139

Cat No. GTX35139

Host Mouse
Clonality Monoclonal
Clone Name TOX3/1123
Isotype IgG2b
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 or 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Specificity/Sensitivity

It recognizes a 63kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.
PROPERTIES

Form

Liquid

Buffer

Prepared in 1mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment (139 Amino acid residues around aa 200-400) of human TOX3 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tox High Mobility Group Box Family Member 3,Cagf9,Tnrc9,Tox3

Background

The protein encoded by this gene contains an HMG-box, indicating that it may be involved in bending and unwinding of DNA and alteration of chromatin structure. The C-terminus of the encoded protein is glutamine-rich due to CAG repeats in the coding sequence. A minor allele of this gene has been implicated in an elevated risk of breast cancer. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES
Anti-TOX3 antibody [TOX3/1123] used in IHC (Paraffin sections) (IHC-P). GTX35139

GTX35139 IHC-P Image

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with TOX3 Monoclonal Antibody (TOX3/1123).

Anti-TOX3 antibody [TOX3/1123] used in IHC (Paraffin sections) (IHC-P). GTX35139

GTX35139 IHC-P Image

Formalin-fixed, paraffin-embedded human Gall Bladder stained with TOX3 Monoclonal Antibody (TOX3/1123).

Anti-TOX3 antibody [TOX3/1123] used in IHC (Paraffin sections) (IHC-P). GTX35139

GTX35139 IHC-P Image

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with TOX3 Monoclonal Antibody (TOX3/1123).

Package List Price ($)
$ 329