GeneTex
United States (US)

TPH2 antibody [N3C3]

GTX101962_41808_20170728_WB_M_hippocampus_19011506_657.jpg
GTX101962_41808_20170728_WB_M_midbrain_19011506_620.jpg
GTX101962_41808_20141017_WB_19011506_962.jpg

Cat No. GTX101962

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HeLa , U87-MG , SK-N-SH , IMR-32 , SK-N-AS , Raw264.7 , C2C12 , mouse hippocampus , mouse midbrain

Predict Reactivity

Rat, Zebrafish, Dog, Chicken, Japanese Medaka, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TPH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ADHD7 antibody, FLJ37295 antibody, MGC138871 antibody, MGC138872 antibody, NTPH antibody, TPH2 antibody, tryptophan 5-monooxygenase 2 antibody, tryptophan 5-hydroxylase 2 antibody, neuronal tryptophan hydroxylase antibody, tryptophan hydroxylase 2 antibody

Background

This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101962_41808_20170728_WB_M_hippocampus_19011506_657.jpg

GTX101962 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101962_41808_20170728_WB_M_midbrain_19011506_620.jpg

GTX101962 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101962_41808_20141017_WB_19011506_962.jpg

GTX101962 WB Image

TPH2 antibody detects TPH2 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody (GTX101962) diluted by 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169