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TPH2 antibody [N3C3]

Anti-TPH2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101962
Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962
Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962
Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

Cat No. GTX101962

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HeLa , U87-MG , SK-N-SH , IMR-32 , SK-N-AS , Raw264.7 , C2C12 , mouse hippocampus , mouse midbrain

Predict Reactivity

Zebrafish, Dog, Chicken, Japanese Medaka, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TPH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tryptophan hydroxylase 2 , ADHD7 , NTPH

Background

This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TPH2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101962

GTX101962 ICC/IF Image

TPH2 antibody [N3C3] detects TPH2 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TPH2 stained by TPH2 antibody [N3C3] (GTX101962) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

GTX101962 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

GTX101962 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

GTX101962 WB Image

TPH2 antibody detects TPH2 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody (GTX101962) diluted by 1:1000.

REFERENCE

There are currently no references for TPH2 antibody [N3C3] (GTX101962). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169