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TPH2 antibody [N3C3]

Anti-TPH2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101962
Anti-TPH2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101962
Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962
Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962
Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962
Anti-TPH2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101962
Anti-TPH2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101962
Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

Cat. No. GTX101962

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all TPH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

mouse hippocampus , mouse midbrain

Predict Reactivity

Dog, Chicken, Japanese Medaka, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TPH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171338

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tryptophan hydroxylase 2 , ADHD7 , NTPH

Background

This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TPH2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101962

GTX101962 ICC/IF Image

TPH2 antibody [N3C3] detects TPH2 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TPH2 stained by TPH2 antibody [N3C3] (GTX101962) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TPH2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101962

GTX101962 IHC-P Image

TPH2 antibody [N3C3] detects TPH2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TPH2 stained by TPH2 antibody [N3C3] (GTX101962) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

GTX101962 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

GTX101962 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

GTX101962 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TPH2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101962

GTX101962 IHC-P Image

TPH2 antibody [N3C3] detects TPH2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TPH2 stained by TPH2 antibody [N3C3] (GTX101962) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TPH2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101962

GTX101962 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using TPH2 antibody [N3C3] (GTX101962) at 1:300 dilution.

Anti-TPH2 antibody [N3C3] used in Western Blot (WB). GTX101962

GTX101962 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TPH2 antibody [N3C3] (GTX101962) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169