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TPK1 antibody

Anti-TPK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103943
Anti-TPK1 antibody used in Western Blot (WB). GTX103943
Anti-TPK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103943
Anti-TPK1 antibody used in Western Blot (WB). GTX103943

Cat. No. GTX103943

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TPK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

H1299 , Mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TPK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952355

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thiamin pyrophosphokinase 1 , HTPK1 , PP20 , THMD5

Background

This gene encodes a protein, that exists as a homodimer, which catalyzes the conversion of thiamine to thiamine pyrophosphate. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TPK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103943

GTX103943 IHC-P Image

TPK1 antibody detects TPK1 protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
TPK1 antibody (GTX103943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TPK1 antibody used in Western Blot (WB). GTX103943

GTX103943 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse liver
12% SDS PAGE
GTX103943 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TPK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103943

GTX103943 IHC-P Image

TPK1 antibody detects TPK1 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TPK1 antibody (GTX103943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TPK1 antibody used in Western Blot (WB). GTX103943

GTX103943 WB Image

TPK1 antibody detects TPK1 protein by western blot analysis.
A. 30 μg H1299 whole cell lysate/extract
12% SDS-PAGE
TPK1 antibody (GTX103943) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

BRADLEY SCOTT HANBERRY et al. 0 2014; TARGETING THE CANCER METABOLIC PHENOTYPE USING HIGH DOSE VITAMIN B1 THERAPY.
Application : WB
Reactivity : Human
Zastre JA et al. J Nutr Biochem 2013; 24 (9) : 1616-24 Up-regulation of vitamin B1 homeostasis genes in breast cancer.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169