GeneTex
United States (US)

TPK1 antibody

GTX103943_39876_WB_19011506_898.jpg
GTX103943_39876_IHC_19011506_720.jpg
GTX103943_39876_IHC_M_19011506_317.jpg
GTX103943_39876_WB_M_Liver_19011506_882.jpg

Cat No. GTX103943

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

H1299 , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TPK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Thiamin Pyrophosphokinase 1,Htpk1,Pp20,Thmd5,Tpk1

Background

This gene encodes a protein, that exists as a homodimer, which catalyzes the conversion of thiamine to thiamine pyrophosphate. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103943_39876_WB_19011506_898.jpg

GTX103943 WB Image

TPK1 antibody detects TPK1 protein by western blot analysis.
A. 30 µg H1299 whole cell lysate/extract
12% SDS-PAGE
TPK1 antibody (GTX103943) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103943_39876_IHC_19011506_720.jpg

GTX103943 IHC-P Image

TPK1 antibody detects TPK1 protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
TPK1 antibody (GTX103943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103943_39876_IHC_M_19011506_317.jpg

GTX103943 IHC-P Image

TPK1 antibody detects TPK1 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TPK1 antibody (GTX103943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103943_39876_WB_M_Liver_19011506_882.jpg

GTX103943 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse liver
12% SDS PAGE
GTX103943 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169