GeneTex
United States (US)

TPP1 antibody, Internal

GTX88372_2405_WB_18121411_830.jpg

Cat No. GTX88372

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88372-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TPSVIRKRYNLTSQD, from the internal region of the protein sequence according to NP_000382.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tripeptidyl Peptidase 1,Cln2,Gig1,Lpic,Scar7,Tpp-1,Tpp1

Cellular Localization

Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Background

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX88372_2405_WB_18121411_830.jpg

GTX88372 WB Image

WB analysis of human placenta lysate using GTX88372 TPP1 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329