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TPP1 antibody [N1C1]

Anti-TPP1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113404
Anti-TPP1 antibody [N1C1] used in Western Blot (WB). GTX113404
Anti-TPP1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113404

Cat No. GTX113404

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TPP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038176

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripeptidyl peptidase 1 , CLN2 , GIG1 , LPIC , SCAR7 , TPP-1

Cellular Localization

Lysosome , Melanosome

Background

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TPP1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113404

GTX113404 IHC-P Image

TPP1 antibody [N1C1] detects TPP1 protein at cytoplasm on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
TPP1 antibody [N1C1] (GTX113404) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TPP1 antibody [N1C1] used in Western Blot (WB). GTX113404

GTX113404 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
7.5% SDS PAGE
GTX113328 diluted at 1:1000

Anti-TPP1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113404

GTX113404 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using TPP1(GTX113404) antibody at 1:200 dilution.

REFERENCE

There are currently no references for TPP1 antibody [N1C1] (GTX113404). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TPP1 antibody [N1C1] (GTX113404). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169