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TPP1 antibody

Anti-TPP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103896
Anti-TPP1 antibody used in Western Blot (WB). GTX103896

Cat No. GTX103896

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TPP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tripeptidyl peptidase 1 , CLN2 , GIG1 , LPIC , SCAR7 , TPP-1

Cellular Localization

Lysosome , Melanosome

Background

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq]

Database

DATA IMAGES
Anti-TPP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103896

GTX103896 IHC-P Image

TPP1 antibody detects TPP1 protein at cytosol on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
TPP1 antibody (GTX103896) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TPP1 antibody used in Western Blot (WB). GTX103896

GTX103896 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
CLN2 antibody
GTX103896 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169