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TPR antibody

Anti-TPR antibody used in Western Blot (WB). GTX130142
Anti-TPR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130142

Cat No. GTX130142

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

267 kDa. ( Note )

Positive Control

mouse lung

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TPR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

translocated promoter region, nuclear basket protein

Cellular Localization

Nucleus , nuclear pore complex , Nucleus membrane; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TPR antibody used in Western Blot (WB). GTX130142

GTX130142 WB Image

TPR antibody detects TPR protein by western blot analysis. Mouse tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with TPR antibody (GTX130142) at a dilution of 1:500.

Anti-TPR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130142

GTX130142 ICC/IF Image

TPR antibody detects TPR protein at nuclear envelope and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TPR protein stained by TPR antibody (GTX130142) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169