GeneTex
United States (US)

TPX2 antibody

GTX115654_40632_IFA_18121410_963.jpg
GTX115654_40632_WB_18121410_241.jpg

Cat No. GTX115654

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TPX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tpx2, Microtubule Nucleation Factor,C20Orf1,C20Orf2,Dil-2,Dil2,Fls353,Gd:C20Orf1,Hca519,Hctp4,Repp86,P100,Tpx2

Cellular Localization

Nucleus , Spindle

Database

Research Area

DATA IMAGES
GTX115654_40632_IFA_18121410_963.jpg

GTX115654 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TPX2(GTX115654) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX115654_40632_WB_18121410_241.jpg

GTX115654 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
7.5% SDS PAGE
GTX115654 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169