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TPX2 antibody

Anti-TPX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115654
Anti-TPX2 antibody used in Western Blot (WB). GTX115654

Cat. No. GTX115654

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TPX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HeLa nucleus

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TPX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162741

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TPX2 microtubule nucleation factor , C20orf1 , C20orf2 , DIL-2 , DIL2 , FLS353 , GD:C20orf1 , HCA519 , HCTP4 , REPP86 , p100

Cellular Localization

Nucleus , Spindle

Database

Research Area

DATA IMAGES

Anti-TPX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115654

GTX115654 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TPX2(GTX115654) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-TPX2 antibody used in Western Blot (WB). GTX115654

GTX115654 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
7.5% SDS PAGE
GTX115654 diluted at 1:1000

REFERENCE

Application Reference

Liu F et al. Adv Sci (Weinh) 2022; 9 (22) : e2104823 A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169