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TRA-2 alpha antibody

Anti-TRA-2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87998
Anti-TRA-2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX87998
Anti-TRA-2 alpha antibody used in Western Blot (WB). GTX87998

Cat. No. GTX87998

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all TRA-2 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human TRA-2 alpha (221-270).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10721933

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AWMS1 , HSU53209 , TRA2A , transformer 2 alpha homolog , TRA-2 alpha , transformer 2 alpha homolog (Drosophila)

Cellular Localization

Nucleus

Background

This gene is a member of the transformer 2 homolog family and encodes a protein with several RRM (RNA recognition motif) domains. This phosphorylated nuclear protein binds to specific RNA sequences and plays a role in the regulation of pre-mRNA splicing. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-TRA-2 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87998

GTX87998 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX87998 TRA-2 alpha antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TRA-2 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX87998

GTX87998 IHC-P Image

IHC-P analysis of human brain tissue using GTX87998 TRA-2 alpha antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TRA-2 alpha antibody used in Western Blot (WB). GTX87998

GTX87998 WB Image

WB analysis of HUVEC cell lysates using GTX87998 TRA-2 alpha antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Yao Lu et al. Front Cell Dev Biol 2022; 10 : 833396 Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B.
Application : WB
Reactivity : Human
Zhu Y et al. Sci Adv 2020; 6 (25) : eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Liu T et al. Mol Cancer Ther 2017; (Epub) tra2a promoted * resistance and tumor progression in triple negative breast cancers via regulating alternative splicing.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399