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TRAF1 antibody, C-term

Anti-TRAF1 antibody, C-term used in Western Blot (WB). GTX89381
Anti-TRAF1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89381
Anti-TRAF1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89381

Cat. No. GTX89381

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all TRAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89381-PEP).

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KLQSPKHAYVKDD, from the C Terminus of the protein sequence according to NP_005649.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor associated factor 1 , EBI6 , MGC:10353

Background

The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-TRAF1 antibody, C-term used in Western Blot (WB). GTX89381

GTX89381 WB Image

WB analysis of U937 cell lysate using GTX89381 TRAF1 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-TRAF1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89381

GTX89381 IHC-P Image

IHC-P analysis of human small intestine using GTX89381 TRAF1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-TRAF1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89381

GTX89381 IHC-P Image

IHC-P analysis of human thymus using GTX89381 TRAF1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399