GeneTex
United States (US)

TRAF2 antibody

KO/KD
GTX16381_20180530_ICCIF_18121410_861.jpg
GTX16381_20180822_WB_18121410_954.jpg
GTX16381_20180530_WB_18121410_120.jpg

Cat No. GTX16381

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

56 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Receptor Associated Factor 2,Mgc:45012,Rnf117,Trap,Trap3,Traf2

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript varia

Database

Research Area

DATA IMAGES
GTX16381_20180530_ICCIF_18121410_861.jpg

GTX16381 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX16381 TRAF2 antibody.
Blue : DAPI

GTX16381_20180822_WB_18121410_954.jpg

GTX16381 WB Image

KO/KD

WB analysis of wild type and TRAF2 knockout cell lysate using GTX16381 TRAF2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX16381_20180530_WB_18121410_120.jpg

GTX16381 WB Image

WB analysis of various samples using GTX16381 TRAF2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 319