GeneTex
United States (US)

TRAF3IP1 antibody [N1N2], N-term

GTX115798_40296_20180817_WB_M_testis_18121410_258.jpg
GTX115798_40296_WB_18121410_857.jpg
GTX115798_40296_IHC_18121410_688.jpg
GTX115798_40296_20180817_WB_R_testis_18121410_704.jpg

Cat No. GTX115798

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

U87-MG , mouse testis , rat testis

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TRAF3IP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Traf3 Interacting Protein 1,Ift54,Mip-T3,Mipt3,Slsn9,Traf3Ip1

Cellular Localization

Cytoplasm , cytoskeleton

Background

Play an inhibitory role on IL13 signaling by binding to IL13RA1. Involved in suppression of IL13-induced STAT6 phosphorylation, transcriptional activity and DNA-binding. Recruits TRAF3 and DISC1 to the microtubules.

Database

Research Area

DATA IMAGES
GTX115798_40296_20180817_WB_M_testis_18121410_258.jpg

GTX115798 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAF3IP1 antibody [N1N2], N-term (GTX115798) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115798_40296_WB_18121410_857.jpg

GTX115798 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX115798 diluted at 1:3000

GTX115798_40296_IHC_18121410_688.jpg

GTX115798 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using TRAF3IP1(GTX115798) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115798_40296_20180817_WB_R_testis_18121410_704.jpg

GTX115798 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAF3IP1 antibody [N1N2], N-term (GTX115798) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169