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TRAF4 antibody

Anti-TRAF4 antibody used in Western Blot (WB). GTX00972
Anti-TRAF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX00972
Anti-TRAF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX00972

Cat No. GTX00972

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml

Note :

IHC-P
Antigen retrieval by heat

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Specificity/Sensitivity

Superfamily members of TRAF4 are not reactive to this antibody.

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF4(437-454aa PKFISHQDIRKRNYVRDD), identical to the related rat and mouse sequences.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CART1,MLN62,RNF83,TNF receptor associated factor 4,TRAF4

Cellular Localization

Cytoplasm,Nucleus,Perinuclear region,Cell junction,Tight junction,Cell membrane,Cytoskeleton

Background

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRAF4 antibody used in Western Blot (WB). GTX00972

GTX00972 WB Image

WB analysis of various sample lysates using GTX00972 TRAF4 antibody.
Lane 1 : Rat Thymus Tissue Lysate
Lane 2 : HeLa Cell Lysate
Lane 3 : Jurkat Cell Lysate
Lane 4: Hepa Cell Lysate

Anti-TRAF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX00972

GTX00972 IHC-P Image

IHC-P analysis of rat lymphonodus tissue using GTX00972 TRAF4 antibody.

Anti-TRAF4 antibody used in IHC (Paraffin sections) (IHC-P). GTX00972

GTX00972 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX00972 TRAF4 antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329