Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.5μg/ml |
0.5-1μg/ml |
Note :
IHC-P
Antigen retrieval by heat
|
Not tested in other applications.
Calculated MW
Product Note
Superfamily members of TRAF4 are not reactive to this antibody.
Form
Liquid
Buffer
5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄
Preservative
0.05mg Thimerosal, 0.05mg Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TRAF4(437-454aa PKFISHQDIRKRNYVRDD), identical to the related rat and mouse sequences.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
CART1 , MLN62 , RNF83 , TNF receptor associated factor 4 , TRAF4
Cellular Localization
Cytoplasm,Nucleus,Perinuclear region,Cell junction,Tight junction,Cell membrane,Cytoskeleton
Background
This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
Database
Research Area