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TRAF6 antibody [N2C2], Internal

Anti-TRAF6 antibody [N2C2], Internal used in Western Blot (WB). GTX113029
Anti-TRAF6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113029
Anti-TRAF6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113029
Anti-TRAF6 antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX113029
Anti-TRAF6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113029
Anti-TRAF6 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113029
Anti-TRAF6 antibody [N2C2], Internal used in Western Blot (WB). GTX113029
Anti-TRAF6 antibody [N2C2], Internal used in Western Blot (WB). GTX113029

Cat. No. GTX113029

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952377

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor associated factor 6 , MGC:3310 , RNF85

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRAF6 antibody [N2C2], Internal used in Western Blot (WB). GTX113029

GTX113029 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:1000.

Anti-TRAF6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113029

GTX113029 IHC-P Image

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRAF6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113029

GTX113029 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using TRAF6(GTX113029) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRAF6 antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX113029

GTX113029 IP Image

TRAF6 antibody [N2C2], Internal immunoprecipitates TRAF6 protein in IP experiments.
IP samples: K562 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TRAF6 protein by 4 μg TRAF6 antibody [N2C2], Internal (GTX113029)
7.5 % SDS-PAGE
The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-TRAF6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113029

GTX113029 IHC-P Image

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRAF6 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113029

GTX113029 ICC/IF Image

TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-TRAF6 antibody [N2C2], Internal used in Western Blot (WB). GTX113029

GTX113029 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: BCL-1
7.5% SDS PAGE
GTX113029 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRAF6 antibody [N2C2], Internal used in Western Blot (WB). GTX113029

GTX113029 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:2000.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169