GeneTex
United States (US)

TRAM1 antibody [C2C3], C-term

GTX108535_WB_19011507_196.jpg
GTX108535_39806_20150116_IHC_M_19011507_139.jpg
GTX108535_39806_IFA_19011507_680.jpg
GTX108535_39806_IHC_19011507_135.jpg

Cat No. GTX108535

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

H1299
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TRAM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Translocation Associated Membrane Protein 1,Pnas8,Tram,Tramp,Tram1

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq]

Database

DATA IMAGES
GTX108535_WB_19011507_196.jpg

GTX108535 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX108535 diluted at 1:1000

GTX108535_39806_20150116_IHC_M_19011507_139.jpg

GTX108535 IHC-P Image

TRAM1 antibody [C2C3], C-term detects TRAM1 protein at cytoplasm on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TRAM1 antibody [C2C3], C-term (GTX108535) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108535_39806_IFA_19011507_680.jpg

GTX108535 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using TRAM1(GTX108535) antibody at 1:500 dilution.

GTX108535_39806_IHC_19011507_135.jpg

GTX108535 IHC-P Image

TRAM1 antibody [C2C3], C-term detects TRAM1 protein at cytoplasm on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
TRAM1 antibody [C2C3], C-term (GTX108535) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169