GeneTex
United States (US)

TRAP antibody

GTX100438_WB_19011506_127.jpg
GTX100438_39959_IHC_19011506_743.jpg
GTX100438_40639_WB_19011506_786.jpg
GTX100438_WB_M_19011506_701.jpg

Cat No. GTX100438

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

A431 , Molt-4 , NIH-3T3

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC117378 antibody, TRAP antibody, ACP5 antibody, tartrate-resistant acid ATPase antibody, tartrate-resistant acid phosphatase type 5 antibody, TrATPase antibody, acid phosphatase 5, tartrate resistant antibody

Cellular Localization

Lysosome

Background

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq]

Database

DATA IMAGES
GTX100438_WB_19011506_127.jpg

GTX100438 WB Image

Sample(30 µg whole cell lysate)
A:A431(GTX27909)
B:MOLT4 (GTX27912)
12% SDS PAGE
GTX100438 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100438_39959_IHC_19011506_743.jpg

GTX100438 IHC-P Image

TRAP antibody detects ACP5 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine tissue.
TRAP antibody (GTX100438) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100438_40639_WB_19011506_786.jpg

GTX100438 WB Image

TRAP antibody detects TRAP protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAP antibody (GTX100438) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100438_WB_M_19011506_701.jpg

GTX100438 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
10% SDS PAGE
GTX100438 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169
Current in productions