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TRAP1 antibody [N1N3]

KO/KD
Anti-TRAP1 antibody [N1N3] used in Western Blot (WB). GTX115352
Anti-TRAP1 antibody [N1N3] used in Western Blot (WB). GTX115352
Anti-TRAP1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115352
Anti-TRAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115352

Cat. No. GTX115352

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

A431 , 293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TRAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166181

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor associated protein 1 , HSP 75 , HSP75 , HSP90L , TRAP-1

Cellular Localization

Mitochondrion

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TRAP1 antibody [N1N3] used in Western Blot (WB). GTX115352

GTX115352 WB Image

KO/KD

Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [N1N3] (GTX115352) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRAP1 antibody [N1N3] used in Western Blot (WB). GTX115352

GTX115352 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX115352 diluted at 1:3000

Anti-TRAP1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115352

GTX115352 IHC-P Image

TRAP1 antibody [N1N3] detects TRAP1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
TRAP1 antibody [N1N3] (GTX115352) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TRAP1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115352

GTX115352 ICC/IF Image

TRAP1 antibody [N1N3] detects TRAP1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec.
Green: TRAP1 protein stained by TRAP1 antibody [N1N3] (GTX115352) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169