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TRAP1 antibody [TRAP1-6]

Anti-TRAP1 antibody [TRAP1-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16195
Anti-TRAP1 antibody [TRAP1-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16195
Anti-TRAP1 antibody [TRAP1-6] used in IHC (Paraffin sections) (IHC-P). GTX16195
Anti-TRAP1 antibody [TRAP1-6] used in Immunoprecipitation (IP). GTX16195

Cat. No. GTX16195

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TRAP1-6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all TRAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000
ICC/IF 1:250
IHC-P 1:10-1:100
IHC-Fr 1:20
IP Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Product Note

Immunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant TRAP1.

Purification

Purified IgG

Conjugation

Unconjugated

RRID

AB_2887526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor associated protein 1 , HSP 75 , HSP75 , HSP90L , TRAP-1

Cellular Localization

Mitochondrion,Mitochondrion inner membrane,Mitochondrion matrix

Background

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-TRAP1 antibody [TRAP1-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16195

GTX16195 ICC/IF Image

ICC/IF analysis of PC-3-M cells using GTX16195 TRAP1 antibody [TRAP1-6].

Anti-TRAP1 antibody [TRAP1-6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16195

GTX16195 ICC/IF Image

ICC/IF analysis of NCI-H460 Cells using GTX16195 TRAP1 antibody [TRAP1-6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-TRAP1 antibody [TRAP1-6] used in IHC (Paraffin sections) (IHC-P). GTX16195

GTX16195 IHC-P Image

IHC-P analysis of human liver tissue using GTX16195 TRAP1 antibody [TRAP1-6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-TRAP1 antibody [TRAP1-6] used in Immunoprecipitation (IP). GTX16195

GTX16195 IP Image

IP analysis of TRAP1 using GTX16195 TRAP1 antibody [TRAP1-6]. The results were visualized by Coomassie Blue staining.

REFERENCE

Application Reference

Lin PH et al. J Biomed Sci 2015; 22 (1) : 44 N-terminal functional domain of Gasdermin A3 regulates mitochondrial homeostasis via mitochondrial targeting.
Application : WB, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399