GeneTex
United States (US)

TRAP1 antibody [TRAP1-6]

GTX16195_350_ICC-IF_19011519_831.jpg
GTX16195_1051_IHC-P_19011519_658.jpg
GTX16195_2053_IP_19011519_540.jpg
GTX16195_349_ICC-IF_19011519_514.jpg

Cat No. GTX16195

Host Mouse
Clonality Monoclonal
Clone Name TRAP1-6
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,000
ICC/IF 1:250
IHC-P 1:10-1:100
IHC-Fr 1:20
IP Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Specificity/Sensitivity

Immunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant TRAP1.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Receptor Associated Protein 1,Hsp 75,Hsp75,Hsp90L,Trap-1,Trap1

Cellular Localization

Mitochondrion,Mitochondrion inner membrane,Mitochondrion matrix

Background

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES
GTX16195_350_ICC-IF_19011519_831.jpg

GTX16195 ICC/IF Image

ICC/IF analysis of PC-3-M cells using GTX16195 TRAP1 antibody [TRAP1-6].

GTX16195_1051_IHC-P_19011519_658.jpg

GTX16195 IHC-P Image

IHC-P analysis of human liver tissue using GTX16195 TRAP1 antibody [TRAP1-6].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX16195_2053_IP_19011519_540.jpg

GTX16195 IP Image

IP analysis of TRAP1 using GTX16195 TRAP1 antibody [TRAP1-6]. The results were visualized by Coomassie Blue staining.

GTX16195_349_ICC-IF_19011519_514.jpg

GTX16195 ICC/IF Image

ICC/IF analysis of NCI-H460 Cells using GTX16195 TRAP1 antibody [TRAP1-6]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

REFERENCE
Package List Price ($)
$ 319