GeneTex
United States (US)

TRAP1 antibody

KO/KD
GTX102017_39694_20180119_WB_KO_watermark_19011506_836.jpg
GTX102017_WB_19011506_431.jpg
GTX102017_39694_WB_M_brain_19011506_129.jpg
GTX102017_39694_IHC_M_19011506_865.jpg
GTX102017_39694_IFA_19011506_315.jpg

Cat No. GTX102017

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Receptor Associated Protein 1,Hsp 75,Hsp75,Hsp90L,Trap-1,Trap1

Cellular Localization

Mitochondrion

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102017_39694_20180119_WB_KO_watermark_19011506_836.jpg

GTX102017 WB Image

KO/KD

Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody (GTX102017) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX102017_WB_19011506_431.jpg

GTX102017 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
B:MOLT4(GTX27912)
7.5% SDS PAGE
GTX102017 diluted at 1:1500

GTX102017_39694_WB_M_brain_19011506_129.jpg

GTX102017 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102017 diluted at 1:1000

GTX102017_39694_IHC_M_19011506_865.jpg

GTX102017 IHC-P Image

TRAP1 antibody detects TRAP1 protein at mitochondrion on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
TRAP1 antibody (GTX102017) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102017_39694_IFA_19011506_315.jpg

GTX102017 ICC/IF Image

TRAP1 antibody detects TRAP1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TRAP1 protein stained by TRAP1 antibody (GTX102017) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169