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TRAP80 antibody [C1C3]

Anti-TRAP80 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115241
Anti-TRAP80 antibody [C1C3] used in Western Blot (WB). GTX115241
Anti-TRAP80 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115241

Cat No. GTX115241

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TRAP80. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619288

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mediator complex subunit 17 , CRSP6 , CRSP77 , DRIP80 , SRB4 , TRAP80

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRAP80 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115241

GTX115241 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MED17(GTX115241) antibody at 1:200 dilution.

Anti-TRAP80 antibody [C1C3] used in Western Blot (WB). GTX115241

GTX115241 WB Image

Sample (30 μg of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX115241 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRAP80 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115241

GTX115241 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MED17(GTX115241) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for TRAP80 antibody [C1C3] (GTX115241). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TRAP80 antibody [C1C3] (GTX115241). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169