*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , Jurkat , Raji
Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the C-terminus region of human TRAP80. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mediator Complex Subunit 17,Crsp6,Crsp77,Drip80,Srb4,Trap80,Med17
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]
GTX115241 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MED17(GTX115241) antibody at 1:200 dilution.
GTX115241 WB Image
Sample (30 µg of whole cell lysate)
7.5% SDS PAGE
GTX115241 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX115241 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MED17(GTX115241) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min