*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , Jurkat , Raji
Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the C-terminus region of human TRAP80. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
mediator complex subunit 17 , CRSP6 , CRSP77 , DRIP80 , SRB4 , TRAP80
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]
GTX115241 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MED17(GTX115241) antibody at 1:200 dilution.
GTX115241 WB Image
Sample (30 μg of whole cell lysate)
7.5% SDS PAGE
GTX115241 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX115241 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MED17(GTX115241) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min