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TRAP80 antibody [C1C3]

Anti-TRAP80 antibody [C1C3] used in Western Blot (WB). GTX115241
Anti-TRAP80 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115241
Anti-TRAP80 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115241

Cat. No. GTX115241

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TRAP80 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TRAP80. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619288

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mediator complex subunit 17 , CRSP6 , CRSP77 , DRIP80 , SRB4 , TRAP80

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRAP80 antibody [C1C3] used in Western Blot (WB). GTX115241

GTX115241 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP80 antibody [C1C3] (GTX115241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRAP80 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115241

GTX115241 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MED17(GTX115241) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRAP80 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115241

GTX115241 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MED17(GTX115241) antibody at 1:200 dilution.

REFERENCE

Application Reference

Dimitrova E et al. Nat Struct Mol Biol 2022; 29 (10) : 1000-1010 Distinct roles for CKM-Mediator in controlling Polycomb-dependent chromosomal interactions and priming genes for induction.
Kawachi T et al. EMBO J 2021; 40 (22) : e107485 Regulated splicing of large exons is linked to phase-separation of vertebrate transcription factors.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169