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TRBP1 antibody

Anti-TRBP1 antibody used in Western Blot (WB). GTX85487

Cat. No. GTX85487

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

182 kDa. ( Note )

Positive Control

3T3 Cell Lysate

Product Note

This antibody is specific for TRBP1 Internal

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TRBP1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human TRBP1.The immunogen is located within amino acids 1310 - 1360 of TRBP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TAR (HIV-1) RNA binding protein 1 , TRM3 , TRMT3 , TRP-185 , TRP185

Background

The human trans-activation response (TAR) RNA binding protein 1 (TRBP1) was initially identified as a protein that binds to the HIV-1 TAR RNA and activates the long terminal repeat (LTR) expression in the absence and presence of the viral trans-activator Tat. This binding is enhanced by the presence of co-factors such as elongation factor 1alpha (EF-1alpha), polypyrimidine tract-binding protein (PTB), and the chaperonin-like protein stimulator of TAR RNA-binding proteins (SRB). TRBP1 may act to disengage RNA polymerase II from TAR during transcriptional elongation if the RNA polymerase stalls at the TAR during transcriptional elongation.

Database

DATA IMAGES

Anti-TRBP1 antibody used in Western Blot (WB). GTX85487

GTX85487 WB Image

WB analysis of 3T3 cell lysate using GTX85487 TRBP1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399