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TRC8 antibody, C-term

Anti-TRC8 antibody, C-term used in Western Blot (WB). GTX88450
Anti-TRC8 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88450
Anti-TRC8 antibody, C-term used in Western Blot (WB). GTX88450

Cat No. GTX88450

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3-4μg/ml

Note :

IHC-P
Human Placenta shows membranous staining in the cytotrophoblasts.

Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88450-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HTGAAAEEFNDDT, from the C Terminus of the protein sequence according to NP_009149.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ring finger protein 139 , HRCA1 , RCA1 , TRC8

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TRC8 antibody, C-term used in Western Blot (WB). GTX88450

GTX88450 WB Image

GTX88450 (1μg/ml) staining of Human Skeletal Muscle lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-TRC8 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88450

GTX88450 IHC-P Image

GTX88450 (3.8μg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-TRC8 antibody, C-term used in Western Blot (WB). GTX88450

GTX88450 WB Image

GTX88450 (0.5μg/ml) staining of Human Skeletal muscle lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 349